After fertilization, GSH plays an active role in the formation of the male pronucleus, and has a beneficial effect on early embryogenesis. 它对于受精后雄原核形成起了积极的作用,并且还有助于早期胚胎发育。
And the third stage displayed one male pronucleus and two female pronuclei taking shape of bulliform structure with treatment duration. 第3种图像可见两个雌性原核和一个雄性原核,随着处理时间延长雌雄原核逐渐形成泡状结构。
Methods Transgenic mice were created by microinjection of α-MHC-HB-EGF construct into male pronucleus of the zygotes. 方法构建α-MHC-HB-EGF表达载体,将目的片段注射到受精卵的雄原核中,使其发育成携带有HB-EGF的转基因小鼠。
After the sperm penetrated into the egg, its nucleus was transformed into a big male pronucleus. 精子入卵后,絮状的精核经过重建形成雄原核。
A preliminary study on the mode in the egg of the naturally gynogenetic crucian carp for inhibiting heterologous sperm from transforming into male pronucleus 雌核发育银鲫卵抑制异源精子原核化的作用模式初探
Pronuclear formation was asynchronous, at 16h and 19h after ICSI 3.2% and 40.0% of oocytes formed male pronucleus, while 71.9% and 88.7% of oocytes formed female pronucleus at 13h and 16h. 雌雄原核发育不同步,雄原核在ICSI后16h和19h的形成率分别为3.2%和40.0%,雌原核在ICSI后13h已达到71.9%,16h时提高到88.7%。
The ooplasm of gynogenetic crucian carp may also lack some high molecules responsible for membrane assembly of male pronucleus. 银鲫卵质中还可能缺乏某些与雄性原核的核膜重组装有关的大分子物质。
The interfering vectors were microinjected into male pronucleus of the embryos. When cultured in vitro, embryo developmental arrest took place in 2 cell stage. It was obvious that the fluorescence of microinjected embryos became weakened under microscope. 小鼠胚胎原核显微注射干扰载体后进行体外培养,发育多数阻滞在二细胞期,此时荧光显微镜下可观察到明显的荧光减弱现象。
In our experiment, 1.3 copy HBV ( genotype D) was used as target gene, which was transferred into male pronucleus of fertilized eggs, and then the DNA-injected fertilized eggs was transplanted into mouse oviduct under phantom pregnancy, which developed into off-spring. 本实验将1.3copy的D基因型HBV全基因组作为目的基因,采用显微注射技术将其注入小鼠受精卵细胞雄原核,然后将受精卵移植于受体假孕母鼠输卵管内,发育产生子代小鼠。